Research Paper Volume 14, Issue 4 pp 1767—1781
The novel lncRNA GPC5-AS1 stabilizes GPC5 mRNA by competitively binding with miR-93/106a to suppress gastric cancer cell proliferation
Figure 5. GPC5-AS1 regulates GPC5 expression by enhancing mRNA stability. (A, B) GPC5 mRNA expression was measured in SGC-7901 and BGC-823 cells transfected with pc-GPC5-AS1. (C) GPC5 protein expression was measured in SGC-7901 and BGC-823 cells transfected with pc-GPC5-AS1. (D, E) GPC5 mRNA and protein expression was measured in SGC-7901 and BGC-823 cells transfected with si-MeCP2. (F, G) GPC5 mRNA expression level was measured at a time curve in SGC-7901 and BGC-823 cells transfected with pc-GPC5-AS1 or ctrl, following treatment with actinomycin D. (p* < 0.05, p** < 0.01).