Research Paper Volume 14, Issue 4 pp 1782—1796

Figure 2. Knockdown of Linc00205 altered cell viability, colony formation, apoptosis, migration and invasion ability. (A) qRT-PCR assay for Linc00205 expression in HepG2 cells that were transfected with either si-Linc00205 or control si-NC. (B) Cell viability assay conducted in HepG2 cells after transfection with either si-Linc00205 or control si-NC. (C) Colony formation ability of HepG2 cells that were transfected with si-Linc00205 or control si-NC. Quantitative analysis of colonies formation (right panel). (D) Apoptosis of HepG2 cells transfected with si-Linc00205 or control si-NC, and quantitative analysis of the apoptosis cells (right panel). (E, F) The migration and invasion ability of HepG2 cells that were transfected with either si-Linc00205 or control si-NC, as determined using the transwell migration assay (E) and transwell invasion assay (F) (left panel). Quantitative analysis of the migratory cells or invasive cells in transwell assays was also conducted (right panel). (G) Migration ability of HepG2 cells transfected with si-Linc00205 or control si-NC at indicated time points was evaluated by wound healing assay (upper panel). Quantitative analysis of the gap of wound healing assay (lower panel) was performed at 48 hours. *p < 0.05, **p < 0.01.