Research Paper Volume 14, Issue 4 pp 1836—1847

Figure 4. PcDNA-ATG4B reversed the KCNQ1OT1 siRNA caused dysregulation of HCT116 and SW480 cells. (A) KCNQ1OT1 siRNA suppressed the expression of KCNQ1OT1 in HCT116 and SW480 cells (statistical analysis: Pupil's t-test; * P value < 0.05 vs. NC siRNA). (B) KCNQ1OT1 siRNA activated the expression of miR-34a in HCT116 and SW480 cells (statistical analysis: Pupil's t-test; * P value < 0.05 vs. NC siRNA). (C) KCNQ1OT1 siRNA suppressed the expression of ATG4B mRNA in HCT116 and SW480 cells (statistical analysis: Pupil's t-test; * P value < 0.05 vs. NC siRNA). (D) KCNQ1OT1 siRNA suppressed the expression of ATG4B protein in HCT116 and SW480 cells (statistical analysis: Pupil's t-test; * P value < 0.05 vs. NC siRNA). (E) PcDNA-ATG4B reversed the KCNQ1OT1 siRNA caused cell viability decrease in HCT116 and SW480 cells. (F) PcDNA-ATG4B reversed the KCNQ1OT1 siRNA caused apoptosis increase in HCT116 and SW480 cells. L-OHP treatment increased the apoptosis level of HCT116 and SW480 cells under distinct conditions (statistical analysis: one-way ANOVA; HCT116: * P value < 0.05, F value = 8.42 vs. NC siRNA; ** P value < 0.05, F value = 7.91 vs. Si KCNQ1OT1; # P value < 0.05, F value = 6.84 vs. NC siRNA + L-OHP; ## P value < 0.05, F value = 11.08 vs. Si KCNQ1OT1 + L-OHP; SW480: * P value < 0.05, F value = 9.99 vs. NC siRNA; ** P value < 0.05, F value = 10.91 vs. Si KCNQ1OT1; # P value < 0.05, F value = 8.79 vs. NC siRNA + L-OHP; ## P value < 0.05, F value = 11.22 vs. Si KCNQ1OT1 + L-OHP).