Research Paper Volume 14, Issue 4 pp 1651—1664
Mitochondrial dysfunction in mandibular hypoplasia, deafness and progeroid features with concomitant lipodystrophy (MDPL) patients
Figure 4. Total and mitochondrial superoxide content after metformin treatment at different concentrations and timing. Flow cytometry quantification of total ROS (A) using CM-H2DCFDA in WT and in POLD1 human dermal fibroblasts after 48 and 72 hours of 40 uM, 100 uM, 500 uM, 1 and 5 mM of metformin treatment. (B) Flow cytometry quantification of mitochondrial superoxide using MitoSOX Red in WT and in POLD1 human dermal fibroblasts after 48 and 72 hours of 40 uM, 100 uM, 500 uM, 1 and 5 mM of metformin treatment.