Research Paper Volume 15, Issue 6 pp 1859—1877

Figure 2. Overexpression of wild-type P53 terminates LR and activates the P53/miR-34a/SIRT1 positive feedback loop early during LR. (A) Representative livers from mice at 7 days after PH between Ade-GFP and Ade-P53 mice. (B) Liver weight relative to body weight at the indicated time points after PH. (C) Representative images of PCNA staining at the indicated time points after PH from the Ade-GFP and Ade-P53 groups (magnification: ×200, scale bars represent 50 μm). (D) Quantification of PCNA-positive cells in the liver at the indicated time points after PH. (E, F) Serum AST and ALT levels in Ade-GFP and Ade-P53 mice after PH. (G) Survival rate of mice that underwent PH from the Ade-GFP and Ade-P53 groups(p=0.047, logrank test). (H) Representative images of TUNEL staining at day 7 after PH in liver tissue (magnification: ×400). (I) Quantification of TUNEL-positive cells in the liver at day 7 after PH. (J) Quantification of hepatic miR-34a expression between Ade-GFP and Ade-P53 mice at day 7 after PH by qPCR. (K) Protein expression of P53/miR-34a/SIRT1 positive feedback loop genes between Ade-GFP and Ade-P53 mice day 7 after PH. (*, p<0.05; **, p<0.01; ***, p<0.001).