Research Paper Volume 15, Issue 6 pp 1859—1877

Figure 3. Knock-down of miR-34a suppresses the P53/miR-34a/SIRT1 positive feedback loop early during LR. (A) Representative livers from mice at 7 days after PH between AAV-GFP and AAV-anti-miR-34a mice. (B) Liver weight relative to body weight at the indicated time points after PH. (C) Representative images of PCNA staining at the indicated time points after PH in the GFP and anti-miR-34a groups (magnification: ×200, scale bars represent 50 μm). (D) Quantification of PCNA-positive cells in the liver at the indicated time points after PH. (E) Representative images of TUNEL staining at day 7 after PH in liver tissue (magnification: ×400). (F) Quantification of TUNEL-positive cells in the liver at day 7 after PH. (G) Protein expression of P53/miR-34a/SIRT1 positive feedback loop genes between AAV-GFP and AAV-miR-34a mice at day 7 after PH. (H) Quantification of hepatic miR-34a expression between AAV-GFP and AAV-miR-34a mice at day 7 after PH by qPCR. (*, p<0.05; **, p<0.01; ***, p<0.001).