Research Paper Volume 15, Issue 6 pp 1859—1877

Figure 5. T-β-MCA facilitates the P53/miR-34a/SIRT1 positive feedback loop during LR by suppressing the FXR/SHP signaling pathway in vivo. (A) Representative livers from mice at 7 days after PH between ND and T-β-MCA mice. Mice in the T-β-MCA group were administered T-β-MCA (400 mg/kg) by gavage 1 day before PH and every 3 days after PH. (B) Liver weight relative to body weight at the indicated time points after PH. (C) Representative images of PCNA staining at the indicated time points after PH from the ND and T-β-MCA groups. (magnification: × 200, Scale bars represent 50 μm). (D) Quantification of PCNA-positive cells in the liver at the indicated time points after PH. (E, F) Serum AST and ALT levels in mice from the ND and MCA groups after PH. (G) Survival rate of mice that underwent PH from the ND and T-β-MCA groups(p=0.045, logrank test). (H) Representative images of TUNEL staining at day 7 after PH in liver tissue (magnification: ×400). (I) Quantification of TUNEL-positive cells in the liver at day 7 after PH. (J) Quantification of hepatic miR-34a expression in mice with/without administration of T-β-MCA. (K) Protein expression of P53/miR-34a/SIRT1 positive feedback loop genes and FXR/SHP signaling with/without administration of T-β-MCA. *, p<0.05. (ns: not significant, p>0.05; *, p<0.05; **, p<0.01; ***, p<0.001).