Research Paper Volume 14, Issue 4 pp 1983—2003

Figure 7. YAP1 as a target gene of the AK4P1/miR-375 axis promotes PDAC progression. (A) Heatmap of DEGs in PDAC tissues compared to normal pancreas tissues were confirmed by microarray. (B) Expression of YAP1 in PDAC and normal pancreas cell lines. (C) Expression of YAP1 in 48 paired PDAC and their adjacent non-cancerous tissues. (D) Expression of YAP1 in PANC-1 cells with stably transfected sh-YAP1-1 and sh-YAP1-2 were detected using qRT-PCR, and the densitometric values of YAP1 expression were analyzed as compared with GAPDH. (E) The effect of knocking down YAP1 on the proliferation of PANC-1 cells, according to the CCK-8 assay. (F) The effect of knocking down YAP1 on the apoptosis rate of PANC-1 cells was determined by flow cytometry. (G) The densitometric analysis of the apoptosis rate of PANC-1 cells after knocking down YAP1 was performed. (H) The effect of knocking down YAP1 on the ability of PANC-1 cells invasion was determined by Transwell assay. (I) The densitometric analysis of the number of the invasion cells after knocking down YAP1 was performed. Data are presented as the means ± SD. Results were one representative of three independent experiments; bars, SD; ^*P < 0.05.