Research Paper Volume 14, Issue 12 pp 5059—5074
The circular RNA hsa_circ_0003091 regulates sepsis-induced lung injury by sponging the miR-149/Smad2 axis
Figure 6. Smad2 is a direct downstream target of miR-149. (A) Putative binding site between Smad2 and miR-149. (B) Representative bar graphs showing the luciferase activity in the cells after indicated treatments. (C) Expression of Smad2 in lungs and isolated ECs. (D) The PMLECs were transfected with mmu_circ_0015268 overexpressing vector (OV) or mmu_circ_0015268 shRNA (shRNA), followed by LPS stimulation. Expression of Smad2 in different groups was measured. (E, F) The mRNA and protein levels of Smad2 in differentially treated PMVECs. **p < 0.01; ***p < 0.001 vs. sham; #p < 0.05 vs. model.