Research Paper Volume 14, Issue 12 pp 5086—5096

Figure 4. Autophagy mediated galectin-3-induced VSMCs calcification. After pre-treatment with 100 mmol/L 3-MA for 1 h, the VSMCs were then treated with 10 μg/ml galectin-3 for 24 h, BMP2, Runx2 and OPN expression were measured by Western blot, the results quantifications were shown in the right panel (A and B). After treatment with Atg5 siRNA for 24 h, the VSMCs were then treated with 10 μg/ml galectin-3 for 24 h, BMP2, Runx2 and OPN expression were measured by Western blot, the results quantifications were shown in the right panel (C and D). Band density of native VSMCs was chosen as a reference and set to 1. Data were obtained from three independent experiments. ^*P < 0.05, vs. the control.