Research Paper Volume 14, Issue 12 pp 5153—5162
Long non-coding RNA NUT family member 2A-antisense RNA 1 sponges microRNA-613 to increase the resistance of gastric cancer cells to matrine through regulating oxidative stress and vascular endothelial growth factor A
Figure 1. The loss of NUTM2A-AS1 attenuates GC tumorigenesis following matrine treatment. (A) The RT-qPCR was employed to evaluate the levels of NUTM2A-AS1 in N87 and AGS cells transfected with sh-NC, sh-NUTM2A-AS1-1, or sh-NUTM2A-AS1-2. ***P < 0.001. (B) The viability of N87 or AGS cells transfected with sh-NC, sh-NUTM2A-AS1-1, or sh-NUTM2A-AS1-2 following matrine treatment was examined by using the MTT assay. *P < 0.05, **P < 0.01. (C) The RT-qPCR was employed to evaluate the levels of NUTM2A-AS1 in N87 and AGS cells transfected with pcDNA3.1 or pcDNA3.1-NUTM2A-AS1. ***P < 0.001. (D) The viability of N87 or AGS cells transfected with pcDNA3.1 or pcDNA3.1-NUTM2A-AS1 following matrine treatment was examined by using the MTT assay. *P < 0.05. (E, F) Cell colonies of N87 and AGS cells transfected with sh-NC, sh-NUTM2A-AS1-1, or sh-NUTM2A-AS1-2 following matrine treatment. Scale bar, 5 μm. ***P<0.001. (G, H) Apoptosis of N87 and AGS cells transfected with sh-NC, sh-NUTM2A-AS1-1, or sh-NUTM2A-AS1-2 following matrine treatment was examined by using the TUNEL assay. Scale bar, 5 μm. ***P < 0.001. (I) The Cancer Genome Atlas analysis of patients with GC showing the NUTM2A-AS1 levels in normal tissues and tumors. *P < 0.05. (J) Kaplan-Meier plot to evaluate the overall survival of patients with GC and high or low NUTM2A-AS1 levels. P = 0.003, high (n = 204), low (n = 204). RT-qPCR, reverse transcription-quantitative polymerase chain reaction; NUTM2A, NUT family member 2A; AS1, antisense RNA 1; GC, gastric cancer; sh, small hairpin; NC, negative control.