Research Paper Volume 14, Issue 12 pp 5153—5162
Long non-coding RNA NUT family member 2A-antisense RNA 1 sponges microRNA-613 to increase the resistance of gastric cancer cells to matrine through regulating oxidative stress and vascular endothelial growth factor A
Figure 3. NUTM2A-AS1 and miR-613 regulate oxidative stress signaling. (A) ROS levels were measured in N87 cells transfected with the NC mimic, miR-613 mimic, NC inhibitor, or miR-613 inhibitor. ***P < 0.001. (B) GSH contents were measured in N87 cells transfected with the NC mimic, miR-613 mimic, NC inhibitor, or miR-613 inhibitor. *P < 0.05, ***P < 0.001. (C) SOD activity was measured in N87 cells transfected with the NC mimic, miR-613 mimic, NC inhibitor, or miR-613 inhibitor. **P < 0.01. (D) ROS levels were measured in N87 cells transfected with sh-NC, sh-NUTM2A-AS1-1, or sh-NUTM2A-AS1-2. ***P < 0.001. (E) GSH contents were measured in N87 cells transfected with sh-NC, sh-NUTM2A-AS1-1, or sh-NUTM2A-AS1-2. ***P < 0.001. (F) SOD activity was measured in N87 cells transfected with sh-NC, sh-NUTM2A-AS1-1, or sh-NUTM2A-AS1-2. *P < 0.05. NUTM2A, NUT family member 2A; AS1, antisense RNA 1; miR, microRNA; sh, small hairpin; NC, negative control; ROS, reactive oxygen species; GSH, glutathione; SOD, superoxide dismutase.