Research Paper Volume 14, Issue 12 pp 4990—5012

Figure 1. RLS is shortened by the loss of Sus1. (A) RLS analysis of four independent sus1Δ strains. The SUS1 allele was replaced with HISMX6 in SY495 and SY652 and KanMX6 in SY653 and SY654. The mean lifespans are shown in parentheses. (B) Growth analysis of the strains used in (C). After spotting cells in 10-fold serial dilutions, the YPD plates were incubated at the indicated temperatures for 2–3 days. (C and D) RLS analysis of the indicated mutants of the SAGA DUB module (C) and TREX-2 (D). The mean lifespans are shown in parentheses. (E) Growth analysis of the strains used in (C and D). After spotting cells in 10-fold serial dilutions, the YPD plates were incubated for 2–3 days at 23°C, 30°C, and 37°C and for 5 days at 16°C.