Research Paper Volume 14, Issue 12 pp 4990—5012

Figure 5. Increased doses of MEX67 or DBP5 restore the mRNA export defect in sus1Δ strains. (A) Representative images of FISH analysis for the WT and sus1Δ strains containing pRS316 expressing the indicated genes. Poly(A)⁺ RNAs were hybridized with Cy3-labeled oligo(dT) probes and visualized via fluorescence microscopy. DNA was stained with DAPI. DIC, DAPI, poly(A)⁺ RNA, and merged images are shown. (B) Quantitative analysis of the FISH results in (A). The nuclear poly(A)⁺ RNA intensity in each cell was divided by the cytoplasmic poly(A)⁺ RNA signal in the corresponding cell. Data are the mean ± SD of triplicate experiments. ^**P < 0.01; ^*P < 0.05; ns, not significant (Student’s t-test between the indicated pairs of values).