Research Paper Volume 14, Issue 17 pp 6905—6916

Figure 6. Suppressing ROS inhibits inflammatory factor expression and NLRP3 activation. (A) Results of cell viability assay (n-3). NAC improved cell viability, which was higher than that in L/N group. ^*P < 0.05, compared with Con group; ^#P < 0.05, compared with L/N group. (B–D) Expression of inflammatory factors (n = 3). NAC down-regulated the expression of inflammatory factors. The levels of IL-6, TNF-α and IL-1β in L/N + NAC group dramatically decreased, lower than those of L/N group. ^*P < 0.05, compared with Con group; ^#P < 0.05, compared with L/N group. (E, F) Detection of protein expression (n = 3). After NAC suppressed ROS, the activation of NLRP3 inflammasome was suppressed, the expression of NLRP3, ASC and Caspase-1 decreased, and the protein levels were dramatically lower than those in L/N group. ^*P < 0.05, compared with Con group; ^#P < 0.05, compared with L/N group.