Research Paper Volume 14, Issue 17 pp 6905—6916

Figure 8. NOX4-KO decreases inflammatory factor expression and NLRP3 inflammasome activation. (A, B) Expression of inflammatory factors in serum and tissues (n = 5). The levels of IL-6, TNF-α and IL-1β in serum and liver tissues of WT-D/L group, higher than those in KO and WT groups while the differences between KO and WT groups were not significant. The inflammatory factor levels in tissues and serum of KO-D/L group declined, lower than those of WT-D/L group. ^*P < 0.05, compared with WT group; ^#P < 0.05, compared with KO group; ^△P < 0.05, compared with WT-D/L group. (C, D) Detection of protein expression (n = 3). NLRP3 was activated in WT-D/L group, and the levels of NLRP3, ASC and Caspase-1 were dramatically higher than those in WT and KO groups. The protein levels in KO-D/L group decreased, lower than those in WT-D/L group. ^*P < 0.05, compared with WT group; ^#P < 0.05, compared with KO group; ^△P < 0.05, compared with WT-D/L group.