Research Paper Volume 14, Issue 19 pp 7794—7823

Figure 3. The expression of IFT88 correlated with INTU expression in LUAD and UCEC samples, and hypermethylation of INTU^CpG was detected in LUAD and UCEC samples. (A, B) The expression of IFT88 positively correlated with the expression of INTU in LUAD (A) and UCEC (B) tumor samples. (C, D) Decreased expression of IFT88 was found associated with reduced OS probabilities in LUAD (C) and UCEC (D) patients. (E) Two putative CpG islands were identified in INTU gene promoter. (F) Two CpG islands were predicted in IFT88 gene promoter. (G, H) Treatment of 5-azacytidine induced INTU expression in NCI-H1975 (G) and AN3 CA (H) cells. n = 3 biological replicates. Each n represents an independent preparation of cell RNA samples. Error bars represent S.E.M. Statistical analysis was performed using two-tailed unpaired Student’s t-test. ^* denotes p < 0.05. (I, J) Treatment of 5-azacytidine induced IFT88 expression in NCI-H1975 (I) and AN3 CA (J) cells. n = 3 biological replicates. Each n represents an independent preparation of cell RNA samples. Error bars represent S.E.M. Statistical analysis was performed using two-tailed unpaired Student’s t-test. ^* denotes p < 0.05. (K) Hypermethylation of the INTU^CpG was detected in LUAD and UCEC tumor samples. (L) No difference in IFT88^CpG methylation levels were detected in LUAD and UCEC samples when compared to their respective normal control samples.