Research Paper Volume 14, Issue 19 pp 7794—7823
Multifaceted investigation underlies diverse mechanisms contributing to the downregulation of Hedgehog pathway-associated genes INTU and IFT88 in lung adenocarcinoma and uterine corpus endometrial carcinoma
Figure 4. TCF4 was identified as a potential transcription factor that mediates INTU and IFT88 downregulation in LUAD and UCEC tumor samples. (A) Nine common transcription factors, including CBFB, HDAC2, JUND, SRF, SUMO2, TAF1, TBP, TCF4 and YY1 were predicted in INTU and IFT88 gene promoters. (B) Illustration of TCF4 binding consensus sequence and the putative TCF4 binding sites in INTU and IFT88 promoter sequence. (C) The TCF4 transcript level was downregulated in LUAD and UCEC tumor samples. * denotes p < 0.05. (D, E) The protein level of TCF4 was downregulated in LUAD (D) and UCEC (E) tumor samples. (F, G) The expression of TCF4 positively correlated with the expression of INTU and IFT88 in LUAD (F) and UCEC (G) tumor samples. (H, I) The LUAD (H) and UCEC (I) patients with lowered level of TCF4 showed reduced OS probabilities.