Research Paper Volume 14, Issue 19 pp 7794—7823
Multifaceted investigation underlies diverse mechanisms contributing to the downregulation of Hedgehog pathway-associated genes INTU and IFT88 in lung adenocarcinoma and uterine corpus endometrial carcinoma
Figure 5. TCF4 interacted with INTU and IFT88 promoters and mediated their gene expression. (A, B) The binding between TCF4 and INTU and IFT88 promoters was detected in NCI-H1975 (A) and AN3 CA (B) cells. n = 3 biological replicates. Each n represents an independent preparation of ChIP samples. Error bars represent S.E.M. Statistical analysis was performed using two-tailed unpaired Student’s t-test. * denotes p < 0.05 and ** denotes p < 0.01. (C, D) Knockdown of TCF4 downregulated the transcript levels of INTU and IFT88 in NCI-H1975 (C) and AN3 CA (D) cells. n = 3 biological replicates. Each n represents an independent preparation of RNA and protein samples. Error bars represent S.E.M. Statistical analysis was performed using two-tailed unpaired Student’s t-test. *denotes p < 0.05, **denotes p < 0.01 and ***denotes p < 0.001.