Research Paper Volume 14, Issue 19 pp 7794—7823
Multifaceted investigation underlies diverse mechanisms contributing to the downregulation of Hedgehog pathway-associated genes INTU and IFT88 in lung adenocarcinoma and uterine corpus endometrial carcinoma
Figure 6. Identification of hsa-miR-212-3p as a communal miRNA against INTU and IFT88 in LUAD and UCEC samples. (A) Identification of hsa-miR-212-3p and hsa-miR-210-3p miRNAs that target both INTU and IFT88 transcripts. (B) Construction of the miRNA-target gene regulatory network. (C, D) The expression of hsa-miR-212-3p negatively correlated with the expression of INTU (C) and IFT88 (D) in LUAD samples. (E, F) The expression of hsa-miR-212-3p negatively correlated with the expression of INTU (E) and IFT88 (F) in UCEC samples. (G, H) Overexpression of hsa-miR-212 led to the downregulation of INTU and IFT88 protein levels in NCI-H1975 (G) and AN3 CA (H) cells. The MALAT1 levels were not affected. n = 3 biological replicates. Each n represents an independent preparation of protein or RNA samples. Error bars represent S.E.M. Statistical analysis was performed using two-tailed unpaired Student’s t-test. ns indicates no significant difference. * denotes p < 0.05.