Results: Bioinformatics analysis revealed that 5 genes were significantly overexpressed in IC/BPS, and the protein-protein interaction diagram showed that BTK was a critical link between these five proteins. At the same time, functional enrichment showed that they were significantly related to innate immunity. Immunoinfiltration showed that mast cell resting in IC/BPS was significantly higher. IHC staining of clinical samples showed that the mast cell markers Tryptase and BTK were highly expressed in IC/BPS tissues. At the cell level, knockdown of BTK inhibited proliferation, migration, invasion, and degranulation of mast cells.

Conclusions: This study provides a new perspective for understanding the molecular mechanisms involved in IC/BPS and suggests that BTK may be a target for treating IC/BPS." name="description"> Bruton tyrosine kinase (BTK) may be a potential therapeutic target for interstitial cystitis/bladder pain syndrome - Figure f1 | Aging

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Research Paper Volume 14, Issue 17 pp 7052—7064

Bruton tyrosine kinase (BTK) may be a potential therapeutic target for interstitial cystitis/bladder pain syndrome

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Figure 1. Identification of DEGs in GEO dataset of IC/BPS patients. (A, B) The ggplot2 package performed principal component analysis (PCA) on the (A) unnormalized and (B) normalized GSE11783, GSE57560 and GSE621 datasets. (C) Volcano maps of the merged datasets. (D) Heatmaps of cluster analysis by DEGs expression in GSE11783, GSE57560 and GSE621 datasets.