Results: Bioinformatics analysis revealed that 5 genes were significantly overexpressed in IC/BPS, and the protein-protein interaction diagram showed that BTK was a critical link between these five proteins. At the same time, functional enrichment showed that they were significantly related to innate immunity. Immunoinfiltration showed that mast cell resting in IC/BPS was significantly higher. IHC staining of clinical samples showed that the mast cell markers Tryptase and BTK were highly expressed in IC/BPS tissues. At the cell level, knockdown of BTK inhibited proliferation, migration, invasion, and degranulation of mast cells.

Conclusions: This study provides a new perspective for understanding the molecular mechanisms involved in IC/BPS and suggests that BTK may be a target for treating IC/BPS." name="description"> Bruton tyrosine kinase (BTK) may be a potential therapeutic target for interstitial cystitis/bladder pain syndrome - Figure f4 | Aging

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Research Paper Volume 14, Issue 17 pp 7052—7064

Bruton tyrosine kinase (BTK) may be a potential therapeutic target for interstitial cystitis/bladder pain syndrome

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Figure 4. Differential expression of BTK in IC/BPS clinical samples. (A) HE staining shows the pathological state of IC/BPS samples. Scale bar: 100 and 50 μm. (B) Masson staining is used to distinguish collagen fibers from muscle fibers. Collagen fibers are blue and smooth muscle fibers are red. The staining results show that compared with NC group, collagen fibers in IC/BPS group are significantly reduced. Scale bar: 100 and 50 μm. (C, D) IHC staining showed the difference of (C) Tryptase and (D) BTK expression in normal and IC/BPS tissues. Scale bar: 20 μm. (E) Western blotting exhibited the expression level of BTK in normal and IC/BPS tissues. In all cases, Values are mean ± SD (n=10 for each group; *P<0.05, **P<0.01).