Research Paper Volume 14, Issue 20 pp 8167—8178
Downregulation of senescence-associated secretory phenotype by knockdown of secreted frizzled-related protein 4 contributes to the prevention of skin aging
Figure 2. Effect of recombinant SFRP4 (rSFRP4) on SASP. (A) Comparison of the percentage of SA-β-gal-positive cells. rSFRP4 treatment increased SA-β-gal expression in proliferating cells. (B) RT-PCR analysis of the SASP gene expression. GAPDH was used as the housekeeping gene. (C) ELISA for protein expression of IL6 and IL8. (D) Western blot analysis of the effects of SFRP4 administration on confirmation and senescence. rSFRP4 treatment was observed to increase SASP at the mRNA and protein levels. rSFRP4; recombinant SFRP4. *; P < 0.05. Mann–Whitney U test was used to analyze differences between two groups. All experiments were performed in triplicate.