Research Paper Volume 14, Issue 19 pp 7866—7876
Comprehensive analysis of DTYMK for estimating the immune microenvironment, diagnosis, prognosis effect in patients with lung adenocarcinoma
Figure 9. DTYMK regulates LUAD cell proliferation, migration and cell cycle. (A) IHC assay detect the protein of DTYMK in lung adenocarcinoma cancerous. (B) qPCR assay examine the expression level of DTYMK in lung adenocarcinoma cancerous cell lines, including A549, H1299, and H1975, compared to normal human bronchial epithelial cell line: BEAS-2B. (C) Establishment of DTYMK knockdown cell lines in H1975 verified by Real-time RT-PCR (D, E) Knockdown of DTYMK significantly inhibits cell proliferation and migration in H1975 cells, as measured by CCK8 and transwell assays. (F) Depletion of DTYMK increased G0/G1 phase arrested cells. NC=negative control, siRNA= DTYMK siRNA ***p < 0.001.