Research Paper Volume 14, Issue 19 pp 7692—7717

Figure 2. Impact of thoracic irradiation on gene expression in lung tissue from three mouse strains with varying susceptibility to fibrosis. Mice were exposed to 5x6 Gy thoracic IR or no IR (0Gy). Samples of lung tissue (n=4 per dose) were collected at 15 weeks after IR, RNA was isolated, and further evaluated with RNA sequencing. (A) Principal component analysis of differentially expressed genes for all evaluated groups (n=4 per condition). (B) Volcano plot of the ratio of gene expression (irradiated: unirradiated) and p value for each observed gene. (C) Unsupervised hierarchical clustering of evaluated samples based on different expression of genes after irradiation. Irradiated samples were evaluated relative to a paired unirradiated sample of the same strain, thus expression depicted is the ratio (irradiated: unirradiated). (D) Hierarchical clustering of samples based on different expression of senescence and aging genes after irradiation. Irradiated samples were evaluated relative to a paired unirradiated sample of the same strain, thus expression depicted is the ratio (irradiated: unirradiated).