Figure 1. AMI model preparation. (A) After anaesthetized with an intraperitoneal injection of 2% pentobarbitone (0.2 ml/100 g), the rat was placed on a temperature control board for the surgical procedures. An intubation cannula connecting with a volume-controlled ventilator was plugged in trachea from its mouth. Three electrodes were subcutaneously placed into the three limbs of rat, and connected to a multi-channel recorder by which the electrocardiogram was recorded. (B) The fur at the left chest was shaved and the skin disinfected with 75% alcohol. (C, D) The echocardiography of rat before and after the coronary ligation; the ST segment elevation could be observed.