Figure 3. Myocardial ischemic area in the 4th subgroup rats of each group. (A–D) (represents the Ctrl, HL, AMI, and HL-AMI group, respectively), Photos of myocardial sections stained by TTC, viable tissue was stained red and infarct area was pale and unstained; (E) Diagram of the myocardial infarction rate of the four groups. MI infarct size was expressed as the percentage of the infarct area relative to the whole myocardium. **, ##, P < 0.01, compared with the AMI, the Ctrl group, respectively. Data are expressed as mean ± SD (n = 8 animals in each group), and vertical lines indicate the SEM.