Research Paper Volume 14, Issue 19 pp 7734—7751

Figure 4. Lipogenesis, beta-oxidation, and the cellular clock in brown adipose tissue during aging. Subscapular brown adipose tissue of young (27–31 weeks old; white circles or bars) and middle-aged (67–71 weeks old; black circles or bars) female APOE*3-Leiden.CETP mice was collected at Zeitgeber Time 0 and 12 (n = 7–8 mice/group/time point). Gene expression of (A) circadian locomotor output cycles kaput (Clock), (B) period 1 (Per1), (C) cryptochrome 1 (Cry1), (D) Nr1d1 (REV-ERBA), (E) brain and muscle ARNT-like 1 (Bmal1), and (F) uncoupling protein 1 (Ucp1) as determined by quantitative polymerase chain reaction, normalized to ß-actin, and shown relative to the expression in young mice at ZT0 (n = 5-8 mice/group/time point). (G) Protein abundance of UCP1 as measured by automated Western blotting, and normalized to Tubulin levels (n = 7–8 mice/group/time point). Gene expression of (H) sterol regulatory element-binding protein 1c (Srebp1c), (I) acetyl-CoA carboxylase (Acc), (J) fatty acid synthase (Fasn), (K) liver X receptor alpha (Lxra), (L) carnitine palmitoyltransferase 1a (Cpt1a), and (M) peroxisomal acyl-coenzyme A oxidase 1 (Acox1). Bar graphs represent means ± SD. ^*p < 0.05; ^**p < 0.01, ^***p < 0.001, according to two-way ANOVA and following Tukey’s multiple-comparison test.