Research Paper Volume 14, Issue 19 pp 7734—7751

Figure 5. Oscillations of lipoprotein in brown adipose tissue and its regulators during aging. Subscapular brown adipose tissue of young (27–31 weeks old; white circles or bars) and middle-aged (67–71 weeks old; black circles or bars) female APOE*3-Leiden.CETP mice was collected at Zeitgeber Time 0 and 12 (n = 7–8 mice/group/time point). (A) Gene expression of lipoprotein lipase (Lpl) measured by quantitative polymerase chain reaction, normalized to ß-actin, and shown relative to the expression in young mice at ZT0. (B) Protein abundance of LPL measured by automated Western blot, and normalized to Tubulin levels. (C) Gene expression of angiopoietin-like 4 (Angptl4). (D) Corticosterone levels measured in plasma collected from young (17–21 weeks old) and middle-aged (57–61 weeks old) mice at Zeitgeber Time 0 and 12. (E) Protein abundance of tyrosine hydroxylase (TH). Bar graphs represent means ± SD. ^**p < 0.01; ^***p < 0.001, according to two-way ANOVA and following Tukey’s multiple-comparison test.