Research Paper Volume 14, Issue 19 pp 7906—7925

Figure 2. UBE2MP1 Depletion impairs cell proliferation and arrests cell cycles in HCC. (A) UBE2MP1 depletion was conducted in HepG2 and Hep3B cells by using shRNA transfection. The RT-qPCR assay demonstrated the significant abrogation of UBE2MP1 transcript in the two treated cell lines, and no significant change of the parental gene UBE2M was induced (**P<0.01). (B, C) The CCK8 assay was conducted to describe cell proliferation status. The cell proliferation in both the two cell lines was significantly suppressed by abrogating the UBE2MP1 transcript (*P<0.05; **P<0.01). (D, E) Flow cytometry was used for analyzing the cell cycle distribution. And the representative histograms were shown. The cell cycle of both HepG2 and Hep3B cells was significantly arrested in G0/G1 phase by depleting the UBE2MP1 transcript. The results are means of three independent experiments ±SD. (*P< 0.05, **P< 0.01).