Research Paper Volume 14, Issue 19 pp 7906—7925
Pseudogene UBE2MP1 derived transcript enhances in vitro cell proliferation and apoptosis resistance of hepatocellular carcinoma cells through miR-145-5p/RGS3 axis
Figure 6. Depletion of miR-145-5p rescued the phenotype induced by UBE2MP1 depletion. (A) The RT-qPCR assay validated the effect of knocking out miR-145-5p in HepG2 cells (**P<0.01). (B) No significant change was induced on the UBE2MP1 transcript by miR-145-5p depleting. (C) The suppressed cell proliferation was significantly recovered by depleting miR-145-5p (*P<0.05, **P<0.01). (D) The arrested cell cycle induced by UBE2MP1 depletion in HepG2 cells was reversed when miR-145-5p was depleted (*P<0.05, **P<0.01). (E) The number of apoptotic cells was decreased in HepG2 cells with UBE2MP1 depletion when RGS3 was elevated (**P<0.01).