Research Paper Volume 14, Issue 20 pp 8321—8345

Jian-Pi-Yi-Shen decoction inhibits mitochondria-dependent granulosa cell apoptosis in a rat model of POF


Figure 10. JPYS inhibited ASK1/JNK-induced apoptosis and mitochondrial dysfunction in vitro. To investigate whether the anti-apoptotic efect of JPYS-containing serum (4 %) was associated with the ASK1/JNK pathway. GS-49977 (5 μM) was the inhibitor of ASK1. (A) We used western blot to detect the target genes of the ASK1/JNK pathway in protein level; (B) The MMP (ratio of red/green); (C) The opening of mPTP (%); (D) The ROS levels; (E) Hoechst 33258 staining was used to detect the apoptosis and counted the percentage of apoptotic cells. Data are shown as mean ± SD. *p < 0.05 versus control group, #p < 0.05 versus CTX group. (n=3).