Research Paper Volume 14, Issue 20 pp 8321—8345

Jian-Pi-Yi-Shen decoction inhibits mitochondria-dependent granulosa cell apoptosis in a rat model of POF


Figure 6. JPYS improved mitochondrial function and inhibited apoptosis via inhibiting ASK1/JNK pathway. Rats were treated with JPYS (11.0 g/kg.d) and pre-treated with triptorelin (1.5 mg/kg) followed by intraperitoneally injected cyclophosphamide (50 mg/kg). Graphical abstract (A): Mitochondrial dysfunction of the ovarian GC occurs following POF and can lead to apoptosis. JPYS can relieve GC apoptosis by improving mitochondrial function via inhibiting the ASK1/JNK pathway in vivo and vitro. We used real-time qPCR and western blot to detect the target genes of ASK1/JNK pathway in mRNA (B) and protein (C) levels. TUNEL positive cells (D), the scale bars represents a length of 50 μm on histology. The activity of caspase-9 (E). The activity of caspase-3 (F). Data are shown as mean ± SD. *p < 0.05 versus control group, #p < 0.05 versus POF group, p < 0.05 versus JPYS group. (n=6).