Research Paper Volume 14, Issue 20 pp 8321—8345

Jian-Pi-Yi-Shen decoction inhibits mitochondria-dependent granulosa cell apoptosis in a rat model of POF


Figure 9. JPYS improved mitochondrial dysfunction caused by CTX in vitro. Cells were treated with CTX (20 μg/ml) for 24 h, then treated with JPYS-containing serum (2, 4, 8 %). (A) Electron microscope pictures (10,000×; 20,000×; 40,000×) of ovary in POF rats, the scale bars represents a length of 2 μm, 1 μm, and 500 nm on cells respectively. Abnormal mitochondrial (paired yellow arrow) morphology show that mitochondrial membrane rupture or swellings, normal mitochondrial (single yellow arrow) morphology type show that mitochondrial membrane smooth and inner carinulae distinct and percentage of damaged mitochondria; (B) The MMP (ratio of red/green); (C) The opening of the mPTP (%); (D) The ROS levels. Data are shown as mean ± SD. *p < 0.05 versus control group, #p < 0.05 versus CTX group, p < 0.05 versus CTX+ JPYS (2 %) group, p < 0.05 versus CTX+ JPYS (4 %) group. (n=3).