Figure 6. Induction of HIF-1α in OM-MSCs with FG-4592 inhibited cerebral OGD/R-induced apoptosis in BV2 microglial cells. (A, B) The successful overexpression of HIF-1α in OM-MSCs was confirmed by Western blotting. (C, E) The apoptosis rate among BV2 microglial cells was determined with flow cytometry and Annexin V/PI staining in each group. (D, F) Production of ROS levels in BV2 microglial cells was measured with flow cytometry. (G, H) Protein expression of caspase3 in BV2 microglial cells was quantified by Western blotting. (I) The viability of BV2 microglial cells was evaluated with MTT assays. All data are presented as the mean ±SD. *p<0.05; **p<0.01, ***p<0.001, compared to the normoxia or hypoxia group.