Research Paper Volume 14, Issue 19 pp 8032—8045
SENP3 affects the expression of PYCR1 to promote bladder cancer proliferation and EMT transformation by deSUMOylation of STAT3
Figure 6. SENP3 promotes tumor proliferation by upregulating STAT3 in vivo. (A) A subcutaneous transplantation tumor model in nude BALB/c mice was established using the human bladder cancer cell line T24. The mice were divided into NC, SENP3(OE) and SENP3(OE) + si-STAT3 groups with 5 mice per group. (B) The growth curve of tumor volumes. [mean ± S.D. (error bars), n = 4. *p ≤ 0.05; **p ≤ 0.01, compared with NC group; ##p ≤ 0.01; ###p ≤ 0.001, compared with STAT3(OE) group, two-way analysis of variance]. (C) PCNA positive cells in subcutaneous transplantation tumor isolates by immunohistochemistry. [scale bar, 50 μm]. (D) PYCR1, E-ca and FN protein level of NC, SENP3(OE) and SENP3(OE) + si-STAT3 mice transplantation tumor.