Research Paper Volume 14, Issue 19 pp 8046—8060
Palmitic acid inhibits vascular smooth muscle cell switch to synthetic phenotype via upregulation of miR-22 expression
Figure 2. PA increased miR-22 expression in VSMCs. (A) MiRNAs levels in VSMCs treated with PA. (B) The mRNA levels of miR-22, miR-23b, and miR-125b in VSMCs after transfecting with their specific miRNA mimics. (C) The protein expression of SM22α, calponin, SMMHC, vimentin, collagen I, and osteopontin in VSMCs treated with miR-22, miR-23b or miR-125b mimics. (D) Migration ability of VSMCs transfecting with miR-122 mimics determined by transwell assay. Scale bar=100 μm. (E, F) The cell proliferation of VSMCs treated with miR-22 mimics detected by EdU assay. (G, H) Apoptosis of VSMCs treated with miR-22 mimics determined by flow cytometry. (I) The protein expression of bax, bcl-2, caspase-3, and cleaved-caspase-3 in VSMCs treated with miR-22 mimics. n = 3. *P < 0.05, **P < 0.01.