Research Paper Volume 14, Issue 19 pp 8046—8060
Palmitic acid inhibits vascular smooth muscle cell switch to synthetic phenotype via upregulation of miR-22 expression
Figure 4. EVI1 was the target of miR-22. (A) Target genes of miR-22 were predicted by miRDB, TargetScan and ENCORI. (B) MRNA levels of target genes in VSMCs treated with miR-22 mimics. (C, D) Protein levels of SM22α, calponin, SMMHC, vimentin, collagen I, and osteopontin in VSMCs detected by western blot. (E) The complementary sequence between 3′-UTR of EVIL1 and miR-22. (F) Dual-luciferase reporter assay. (G) EVI1 protein level was detected by western blot in VSMCs treated with miR-22 inhibitor or PA. n = 3. *P < 0.05, **P < 0.01.