Research Paper Volume 14, Issue 19 pp 8046—8060
Palmitic acid inhibits vascular smooth muscle cell switch to synthetic phenotype via upregulation of miR-22 expression
Figure 5. PA treatment enhanced miR-22 mimic impacts on aborted the inhibitive effect of EVI1 on VSMC phenotype switch. (A) Expression of miR-22 and EVIL1 in VMSCs treated with EVI1 or/and miR-22 overexpression followed by PA treatment. (B) Cell proliferation was detected by EdU in VSMCs with EVI1/miR-22 overexpression followed by PA treatment. (C) Flow cytometry to detect the apoptosis of VSMCs with EVI1/miR-22 overexpression followed by PA treatment. (D) Western blot to detect the apoptosis associated markers in VSMCs. (E, F). Cell migration was detected by transwell assay in EVI1/miR-22-overexpressed VSMCs followed by PA treatment. Scale bar = 100 μm. (G) Protein levels of SM22α, calponin, SMMHC, vimentin, collagen I, and osteopontin in VSMC in VSMCs with EVI1/miR-22 overexpression followed by PA treatment detected by western blot. n = 3. *P < 0.05, **P < 0.01.