Research Paper Volume 14, Issue 20 pp 8221—8242
AR12 increases BAG3 expression which is essential for Tau and APP degradation via LC3-associated phagocytosis and macroautophagy
Figure 10. Degradation of chaperones and eIF2α S51 phosphorylation requires LAP and macroautophagy. (A) HCN2 and (B) BV2 cells were transfected with a scrambled siRNA or with siRNA molecules to knock down the expression of Rubicon, Beclin1 or ATG5. After 24h, cells were treated with vehicle control, AR12 (2 μM), neratinib (50 nM) or the drugs in combination for 6h. Cells were fixed in place and immunostaining performed to determine the expression of GRP78 (total and cell surface / plasma membrane), HSP70, HSP90, eIF2α and ERK2, and the phosphorylation of eIF2α S51. (n = 3 +/-SD) * p < 0.05 less than vehicle control; # p < 0.05 greater than vehicle control.