Research Paper Volume 14, Issue 20 pp 8221—8242
AR12 increases BAG3 expression which is essential for Tau and APP degradation via LC3-associated phagocytosis and macroautophagy
Figure 3. Knock down of Beclin1 or ATG5 prevents autophagosome formation and autophagic flux in HCN2 cells. HCN2 neuronal cells were transfected with a scrambled siRNA or with an siRNA to knock down the expression of ATG5 or Beclin1 and were co-transfected with a plasmid to express LC3-GFP-RFP. After 24h, cells were treated with vehicle control, AR12 (2 μM), neratinib (50 nM) or the drugs in combination for 4h and 8h. The mean number of intense GFP+RFP+ and RFP+ punctae per cell was determined (n = 3 +/-SD) # p < 0.05 greater than vehicle control; ## p < 0.05 greater than AR12 alone value; † p < 0.05 less than corresponding value in siSCR cells; ∞ p < 0.05 greater than corresponding value at the 4h timepoint.