Research Paper Volume 14, Issue 20 pp 8221—8242

Figure 6. Expression of Tau or APP does not significantly alter the regulation of protein phosphorylation or protein expression caused by AR12 and neratinib. HCN2 cells were transfected with an empty vector plasmid or with plasmids to express Tau or APP. Twenty-four h afterwards, cells were treated with vehicle control, AR12 (2 μM), neratinib (50 nM) or the drugs in combination for 6h. Cells were fixed in place and immunostaining performed to determine the phosphorylation and expression of the indicated proteins (n = 3 +/-SD). * p < 0.05 less than vehicle control; # p < 0.05 greater than vehicle control. All expression / phosphorylation levels were normalized to vehicle control cells transfected with the empty vector plasmid.