Research Paper Volume 14, Issue 20 pp 8221—8242
AR12 increases BAG3 expression which is essential for Tau and APP degradation via LC3-associated phagocytosis and macroautophagy
Figure 7. AR12 and neratinib cause degradation of p70 S6K in HCN2 neuronal cells. (A) HCN2 cells were transfected with a scrambled siRNA control or with an siRNA to knock down expression of AMPKa. After 24h, cells were treated with vehicle control or with [AR12 (2 μM) + neratinib (50 nM)] for 6h. Cells were fixed in place and immunostaining performed to determine the phosphorylation and expression of the indicated proteins (n = 3 +/-SD). * p < 0.05 less than vehicle control; # p < 0.05 greater than vehicle control. (B) HCN2 cells were transfected with a scrambled siRNA control or with siRNA molecules to knock down expression of either Beclin1 or ATG5. After 24h, cells were treated with vehicle control or with [AR12 (2 μM) + neratinib (50 nM)] for 6h. Cells were fixed in place and immunostaining performed to determine the phosphorylation and expression of the indicated proteins (n = 3 +/-SD). * p < 0.05 less than vehicle control; # p < 0.05 greater than vehicle control.