Research Paper Volume 14, Issue 20 pp 8346—8356
Parthenolide targets NF-κB (P50) to inhibit HIF-1α-mediated metabolic reprogramming of HCC
Figure 4. Silencing P50 inhibits the effect of Par. (A–C) DMSO-RNAi+Par group differed insignificantly from DMSO-RNAi group regarding the numbers of invasive and metastatic cells. nsP > 0.05 vs. DMSO-RNAi group. (D–F) In the glycolysis assay, the glucose uptake, intracellular glucose level and lactate expression of the DMSO-RNAi+Par group differed insignificantly from those of the DMSO-RNAi group. nsP > 0.05 vs. DMSO-RNAi group. (G) Insignificant difference in the number of plate clones was noted between the DMSO-RNAi+Par and the DMSO-RNAi groups. (H, I) After P50 silencing, Par exerted an insignificant effect on the HIF-1α expression. nsP > 0.05 vs. DMSO-RNAi group. (J, K) Difference in the Brdu-positive cell count was insignificant between the DMSO-RNAi+Par and the DMSO-RNAi groups.