Research Paper Volume 14, Issue 20 pp 8270—8291

Figure 1. Constant expression of OKSM, Sen and OKSM-Sen led to increased stem cell proliferation over time. (A) esgGal4, UAS-GFP; tubGal80^ts> UAS-TdTomato control flies show a small number of stem cells (n < 10) and few enteroblasts during after seven days. Expression of OKSM (B), Sen (C) or both Sen and OKSM (D) led to an increase in both ISCs and EBs with the highest number of ISCs observed in the Sen condition as quantified (Right). On day 14, little change was observed in control files (E), but consistently higher numbers of ISCs were maintained in all three experimental conditions (F–H, quantified Right). Day 21 showed little change from day 14 with similar numbers of ISCs observed in the control flies (I) and consistently higher numbers in the three experimental conditions (J–L, quantified Right). By day 28, the number of ISCs was markedly decreased in control flies (M), while all three experimental conditions maintained ISC numbers (N–P, quantified Right).