Research Paper Volume 14, Issue 20 pp 8292—8301

Figure 1. αS overexpression and toxicity. (A) SH-SY5Y neuroblastoma cells were transfected by human αS plasmid. (B) SH-SY5Y cells were transiently transfected by αS, and post-transfection incubated for an additional 24 h and subjected to test cell viability by DNA laddering and nucleic staining by DAPI (C) where the whitish inclusions (cropped) indicated apoptotic cells with nuclear pyknosis; the pyknosis rate (% chromatin aggregation) was calculated by the scoring percent of cells with nuclear pyknosis in total DAPI-stained cells (scale bar = 40 μm). ^###p < 0.001 compared between vector (vec) vs. αS^+/+.