Research Paper Volume 14, Issue 21 pp 8745—8762
Inhibition of NLRP3 and Golph3 ameliorates diabetes-induced neuroinflammation in vitro and in vivo
Figure 1. NLPR3 involves HG induced GA stress. (A) WT BV2 cells were treated with or without HG (35 mM) for 12h; WT BV2 cells were treated with nocodazole (10 μg/kg) for 5h, NLRP3 KO BV2 cells were treated with or without HG for 12 h, GM130 was detected by immunofluorescence. (B) The percent of cells with dispersed Golgi apparatus normalized with total cells were calculated. Bar = 2 μm. Data represent means ± SEM of 3 independent experiments. * p ≤ 0.05, ** p ≤ 0.01, and *** p ≤ 0.001 according to two-way ANOVA with Bonferroni’s post hoc test.