Research Paper Volume 14, Issue 21 pp 8745—8762

Figure 5. Combination of NLRP3 inhibitor and Golph3 inhibitor ameliorates STZ-induced diabetes neuroinflammation. Diabetes mice were constructed by injection of STZ at 70 mg/kg every other day for 3 times, the body weight and blood glucose were record every week for 4 weeks, then the mice were given ZAF, BRO, or ZAF and BRO, the mice in control group were given DMSO every day for 5 weeks, the body weight and blood glucose were record every week for 9 weeks (A, B). (C) Average time spent to locate submerged escape platform during the 7-d training session in the Morris water maze (n = 8/group). (D) Average time spent on the platform and in the four quadrant areas when the platform was absent (n = 8/group). (E) Average speed spent on the platform and in the four quadrant areas when the platform was absent (n=8/group). (F) Average numbers of mice crossing over the platform area(n=8/group). (G) Representative paths of mice on platform and in the four quadrant areas when the platform was absent (n = 8/group). (H, I) qPCR detection of the IL-1β and IL6 transcription level of mice hippocampus tissue treated with ZAF, BRO, and combination of ZAF and BRO. (J, K) ELISA detection of IL-1β and IL6 in mice hippocampus tissue supernatant treated with ZAF, BRO, and combination of ZAF and BRO. (L) Western blot analysis of NLRP3, caspase1 and IL-1β mice hippocampus tissue treated with ZAF, BRO, and combination of ZAF and BRO. (M) Western blot analysis of Golph3, GM130, Vimentin in mice hippocampus tissue treated with ZAF, BRO, and combination of ZAF and BRO. GAPDH was used as an internal control for normalization. Data represent means ± SEM of 3 independent experiments. * p ≤ 0.05, ** p ≤ 0.01, and *** p ≤ 0.001 according to two-way ANOVA with Bonferroni's post hoc test.