Research Paper Volume 14, Issue 21 pp 8633—8644
Epigallocatechin-3-gallate protects porcine oocytes against post-ovulatory aging through inhibition of oxidative stress
Figure 5. Epigallocatechin-3-gallate (EGCG) rescues apoptosis and autophagy induced by oocyte aging. (A) Active-caspase 3 expression was analyzed by immunofluorescence in the Fresh, 24 h control, 24 h EGCG, 48 h control, and 48 h EGCG oocytes. (B) Beclin 1 level was analyzed by immunofluorescence in the Fresh, 24 h control, 24 h EGCG, 48 h control, and 48 h EGCG oocytes. (C) P53 expression was analyzed by immunofluorescence in the Fresh, 24 h control, 24 h EGCG, 48 h control, and 48 h EGCG oocytes. (D) Relative intensities of caspase 3, Beclin 1, and P53 in the Fresh, 24 h control, 24 h EGCG, 48 h control, and 48 h EGCG oocytes. Scale bars represent 20 μm. *p < 0.05, **p < 0.01 indicate significant differences between treatment groups. Values are expressed as the mean ± SEM.