Research Paper Volume 14, Issue 21 pp 8645—8660
The deubiquitylase USP7 is a novel cyclin F-interacting protein and regulates cyclin F protein stability
Figure 4. USP7 regulates cyclin F mRNA levels. (A) HeLa cells treated with DMSO or P22077 (25 μM) for the indicated hours were collected and total RNA was extracted. cDNAs prepared from each sample were amplified using primer pairs specific to each gene as indicated. The PCR products were analyzed by agarose gel electrophoresis and stained using ethidium bromide. RPL35A was the loading control. Fold change in cyclin F:RPL35A ratios were determined using densitometry values of the amplicons. The values represent an average from two independent experiments. (B) HCT116 cells treated with DMSO or P22077 (25 μM) for 4 h were analyzed as in (A).